Genetic Techniques for Biological Research

Genetic Techniques for Biological Research

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qWe must teach students the conceptual and technical approaches used to solve problems in biology. This book will do this superbly.q - Professor Steve Oliver, School of Biological Sciences, University of Manchester, UK Molecular genetic analysis is the fusion of biochemical and genetic approaches to investigate the underlying mechanisms of cellular processes. These techniques have been developed over the past decades by researchers working with model organisms such as bacteriophage, E. coli, Saccharomyces, Drosophila, maize, and more recently, Arabidopsis and C. elegans. The details of cultivation and crossing of each organism differ, but the theory behind molecular genetic analysis is the same for all of these organisms. Genetic Techniques for Biological Research teaches the methods of molecular genetic analysis using a case study approach with the yeast Saccharomyces as the model genetic organism. The tools available for Saccharomyces are the most highly developed, and yet also most straightforward, of all of the eukaryotic research organisms. Furthermore, the theoretical basis of the methods of molecular genetic analysis learned using yeast are directly applicable to other organisms. The aim is that the reader will develop an appreciation for the molecular genetic method of analysis, and will be able to translate the critical thinking skills illustrated by the case studies to the reader's own research projects and scientific decision making. Features include: * theoretical overviews of the methods and tools of molecular genetic analysis * the application of these techniques to published research articles * case studies with questions to enable students to develop critical thinking skills Genetic Techniques for Biological Research teaches the theory and practice of molecular genetic analysis to advanced undergraduates and graduates studying genetics, genomics, molecular biology and cell biology and is a valuable addition to the reference library of any research laboratory.Additional reading material and techniques manuals are listed at the end of the chapter for those needing more information. CELL FRACTIONATION ... A French press is used for large-sized samples (several grams of cells). This device forces anbsp;...

Title:Genetic Techniques for Biological Research
Author: Corinne A. Michels
Publisher:John Wiley & Sons - 2002-06-10

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